我們的試劑品牌很多,都是高品質(zhì)的試劑

德國(guó)原裝進(jìn)口Marienfeld血球計(jì)數(shù)板/血球計(jì)算盤(pán)/血細(xì)胞計(jì)數(shù)板Dark-Line(0650010暗線),可以重復(fù)使用。Neubauer-improved Counting chambers with V-slash由特殊光學(xué)玻璃制成,計(jì)算區(qū)位于中央,每一計(jì)算盤(pán)有2塊計(jì)算區(qū)改進(jìn)Neubauer improved Dark-Line(0650010)為暗線透明底刻畫(huà)深度為0.1mm,每格面積為0.0025平方毫米計(jì)算盤(pán)中央部分的表面經(jīng)過(guò)特殊加工拋光適用于紅、白血球與血小板的分析,亦適用于細(xì)胞計(jì)算包裝規(guī)格:每只塑料盒內(nèi)含1塊計(jì)數(shù)板與2片專(zhuān)用蓋玻片(22*26*0.4mm)。細(xì)胞計(jì)數(shù)板與專(zhuān)用蓋玻片皆可重復(fù)使用。

安全信息符號(hào)信號(hào)詞危險(xiǎn)聲明警示性聲明危害碼(歐洲)風(fēng)險(xiǎn)聲明(歐洲)安全聲明(歐洲)RIDADRNONH for all modes of transportWGK德國(guó)RTECS閃點(diǎn)(華氏)閃點(diǎn)(攝氏)

產(chǎn)品特性級(jí)別low dust描述anionic detergent含量≥99.5%摩爾質(zhì)量288.38 g/mol應(yīng)用HPLC: suitable熔點(diǎn)204-207 °C (lit.)應(yīng)用陰離子去垢劑十二烷基硫酸鈉作為一種測(cè)試化合物,用于研究解淀粉芽孢桿菌KPS46是否可誘導(dǎo)大豆(Glycine max L.)反抗幾種疾病的啟動(dòng)、信號(hào)傳導(dǎo)、蛋白質(zhì)生產(chǎn)以及系統(tǒng)保護(hù)。生物化學(xué)/生理學(xué)行為十二烷基硫酸鈉(SDS)是通過(guò)疏水作用與蛋白質(zhì)形成復(fù)合物的陰離子去垢劑。 研究表明蛋白質(zhì) - SDS復(fù)合物的流體動(dòng)力學(xué)功能取決于他們多肽鏈的長(zhǎng)度。 因此,基于SDS的電泳技術(shù)可以基于其分子量分離蛋白質(zhì)分子。此外,SDS可以溶解細(xì)胞膜,并且可以提取膜結(jié)合蛋白。分析注意事項(xiàng)針對(duì)用于變性聚丙烯酰胺凝膠電泳進(jìn)行了測(cè)試。

產(chǎn)品特性級(jí)別low dust描述anionic detergent含量≥99.5%摩爾質(zhì)量288.38 g/mol應(yīng)用HPLC: suitable熔點(diǎn)204-207 °C (lit.)應(yīng)用陰離子去垢劑十二烷基硫酸鈉作為一種測(cè)試化合物,用于研究解淀粉芽孢桿菌KPS46是否可誘導(dǎo)大豆(Glycine max L.)反抗幾種疾病的啟動(dòng)、信號(hào)傳導(dǎo)、蛋白質(zhì)生產(chǎn)以及系統(tǒng)保護(hù)。生物化學(xué)/生理學(xué)行為十二烷基硫酸鈉(SDS)是通過(guò)疏水作用與蛋白質(zhì)形成復(fù)合物的陰離子去垢劑。 研究表明蛋白質(zhì) - SDS復(fù)合物的流體動(dòng)力學(xué)功能取決于他們多肽鏈的長(zhǎng)度。 因此,基于SDS的電泳技術(shù)可以基于其分子量分離蛋白質(zhì)分子。此外,SDS可以溶解細(xì)胞膜,并且可以提取膜結(jié)合蛋白。分析注意事項(xiàng)針對(duì)用于變性聚丙烯酰胺凝膠電泳進(jìn)行了測(cè)試。

產(chǎn)品特性pH值5 - 7特色行業(yè)Agriculture灰分<=0.1 %級(jí)別for molecular biology痕量陽(yáng)離子Fe: <=1 ppm熔點(diǎn)118 - 121 °C(lit.)含量>=99.5%雜質(zhì)<=0.3% water外源活性位點(diǎn)DNase, RNase, none detected儲(chǔ)存溫度常溫應(yīng)用離液劑和強(qiáng)變性劑;溶解細(xì)胞。

產(chǎn)品特性可溶性H2O: soluble6 M, clear (100 Hazen)熔點(diǎn)180-185°C(lit.)含量>=99.5%應(yīng)用強(qiáng)離液劑,可用于蛋白質(zhì)的變性和隨后的復(fù)性。 這種強(qiáng)變性劑可以溶解不溶性或變性的蛋白質(zhì),例如包涵體。 這可以用作將蛋白質(zhì)或酶重折疊成其活性形式的第一步。 尿素和二硫蘇糖醇(DTT)也可能是必需的。

產(chǎn)品特性可溶性H2O: soluble6 M, clear (100 Hazen)熔點(diǎn)180-185°C(lit.)含量>=99.5%應(yīng)用強(qiáng)離液劑,可用于蛋白質(zhì)的變性和隨后的復(fù)性。 這種強(qiáng)變性劑可以溶解不溶性或變性的蛋白質(zhì),例如包涵體。 這可以用作將蛋白質(zhì)或酶重折疊成其活性形式的第一步。 尿素和二硫蘇糖醇(DTT)也可能是必需的。

產(chǎn)品特性HLB15轉(zhuǎn)變溫度cloud point 65°C摩爾質(zhì)量average mol wt 1310摩爾質(zhì)量micellar avg mol wt 79,000CMC0.012 mM(20-25C)形態(tài)viscous liquid組成Oleic acid, >=58.0% (balance primarily linoleic, palmitic, and stearic acids)描述non-ionic聚集數(shù)60應(yīng)用非離子型洗滌劑,用于從哺乳動(dòng)物細(xì)胞系的細(xì)胞核中選擇性提取和分離蛋白質(zhì)。

產(chǎn)品特性HLB15轉(zhuǎn)變溫度cloud point 65°C摩爾質(zhì)量average mol wt 1310摩爾質(zhì)量micellar avg mol wt 79,000CMC0.012 mM(20-25C)形態(tài)viscous liquid組成Oleic acid, >=58.0% (balance primarily linoleic, palmitic, and stearic acids)描述non-ionic聚集數(shù)60應(yīng)用非離子型洗滌劑,用于從哺乳動(dòng)物細(xì)胞系的細(xì)胞核中選擇性提取和分離蛋白質(zhì)。

產(chǎn)品特性HLB15轉(zhuǎn)變溫度cloud point 65°C摩爾質(zhì)量average mol wt 1310摩爾質(zhì)量micellar avg mol wt 79,000CMC0.012 mM(20-25C)形態(tài)viscous liquid組成Oleic acid, >=58.0% (balance primarily linoleic, palmitic, and stearic acids)描述non-ionic聚集數(shù)60應(yīng)用非離子型洗滌劑,用于從哺乳動(dòng)物細(xì)胞系的細(xì)胞核中選擇性提取和分離蛋白質(zhì)。

產(chǎn)品簡(jiǎn)介:SDS-PAGE 上樣緩沖液(SDS-PAGE Loading Buffer, 5X),是一種經(jīng)過(guò)改良的以溴酚藍(lán)為染料,5 倍濃縮的蛋白上樣緩沖液,用于 SDS-PAGE 時(shí)蛋白樣品的上樣操作。不含傳統(tǒng)的 β-巰基乙醇或 DTT,Genefist 上樣緩沖液采用 TCEP 作為還原劑,穩(wěn)定性更好、還原效果更優(yōu),并且無(wú)難聞氣味!保存條件:-20℃保存,至少一年有效。使用說(shuō)明:1、在室溫或不超過(guò) 37℃的水浴中溶解蛋白上樣緩沖液。水浴溶解后立即室溫存放,盡量避免長(zhǎng)時(shí)間置于水浴中。使用完畢后置于-20℃保存。2、按照每 4ul 蛋白樣品加入 1ul 上樣緩沖液的比例,混合蛋白樣品和上樣緩沖液。3、100℃或沸水浴加熱 5-10 分鐘,以充分變性蛋白。4、冷卻到室溫后,上樣到 SDS-PAGE 膠加樣孔內(nèi),開(kāi)始電泳。注意事項(xiàng):? 由于含有變性劑,本品不適用于非變性膠的電泳? SDS-PAGE 蛋白上樣緩沖液(5X)必須完全溶解后再使用。? 如果起始時(shí)細(xì)胞或組織的用量較大,基因組 DNA 含量較高,“混合液”可適當(dāng)延長(zhǎng)加熱時(shí)間。? 本產(chǎn)品僅限于專(zhuān)業(yè)人員的科學(xué)研究用,不得用于臨床診斷或治療。

產(chǎn)品簡(jiǎn)介通用型抗體稀釋液是一種即用型的,適合于稀釋所有免疫抗體測(cè)定(Immunofluorescence, IHC, ELISA, WB)實(shí)驗(yàn)中的抗體。該試劑可用于一抗或二抗的稀釋和配制,在 4℃保存和使用不少于六個(gè)月。稀釋液中加入了多種抗體結(jié)合反應(yīng)增強(qiáng)劑及穩(wěn)定劑,能夠增加抗體的溶解度和穩(wěn)定性,增強(qiáng)免疫反應(yīng),降低非特異的背景顏色,提高實(shí)驗(yàn)的特異性和靈敏度,可反復(fù)使用,節(jié)約寶貴的抗體。試劑中不含有動(dòng)物源的蛋白,適合于所有類(lèi)型抗體稀釋?zhuān)?HRP,AP 標(biāo)記的抗體。不含磷酸鹽、疊氮鈉或硫柳汞類(lèi)防腐劑,安全性更高,。產(chǎn)品特色? 即用型稀釋液,無(wú)需配制? 稀釋后的抗體可以使用長(zhǎng)達(dá) 6 個(gè)月? 適用所有實(shí)驗(yàn)的抗體稀釋?zhuān)↖F、IHC、ELISA、WB)? 含抗體結(jié)合反應(yīng)增強(qiáng)劑、穩(wěn)定劑,效果更佳? 不含 BSA、脫脂奶粉等動(dòng)物源蛋白? 不含磷酸鹽、疊氮鈉或硫柳汞類(lèi)防腐劑運(yùn)輸和保存條件常溫運(yùn)輸,4℃存儲(chǔ),有效期兩年。操作步驟 :? 根據(jù)抗體使用說(shuō)明書(shū),直接用本品對(duì)一抗或二抗進(jìn)行適當(dāng)倍數(shù)的稀釋。? 保存在抗體稀釋液中的抗體可回收于 4℃保存,可反復(fù)使用多次,長(zhǎng)達(dá) 6 個(gè)月以上,直至不能達(dá)到理想結(jié)果后丟棄。注意事項(xiàng)1、使用后請(qǐng)旋緊瓶蓋,防止溶液揮發(fā)或與空氣的物質(zhì)發(fā)生化學(xué)反應(yīng),并放回 4℃保存。2、在進(jìn)行抗體免疫反應(yīng)前可以使用 Genefist 的高效 Western 封閉液(貨號(hào)GF1815)進(jìn)行封閉,能進(jìn)一步提高條帶的清晰度,減少顯色后的高背景和雜帶的產(chǎn)生 。3、本品不含疊氮鈉或硫柳汞類(lèi)防腐劑,但還需小心操作避免皮膚直接接觸。4、本試劑只能用于科研實(shí)驗(yàn),請(qǐng)勿用于臨床診斷和其它用途。

產(chǎn)品簡(jiǎn)介通用型抗體稀釋液是一種即用型的,適合于稀釋所有免疫抗體測(cè)定(Immunofluorescence, IHC, ELISA, WB)實(shí)驗(yàn)中的抗體。該試劑可用于一抗或二抗的稀釋和配制,在 4℃保存和使用不少于六個(gè)月。稀釋液中加入了多種抗體結(jié)合反應(yīng)增強(qiáng)劑及穩(wěn)定劑,能夠增加抗體的溶解度和穩(wěn)定性,增強(qiáng)免疫反應(yīng),降低非特異的背景顏色,提高實(shí)驗(yàn)的特異性和靈敏度,可反復(fù)使用,節(jié)約寶貴的抗體。試劑中不含有動(dòng)物源的蛋白,適合于所有類(lèi)型抗體稀釋?zhuān)?HRP,AP 標(biāo)記的抗體。不含磷酸鹽、疊氮鈉或硫柳汞類(lèi)防腐劑,安全性更高,。產(chǎn)品特色? 即用型稀釋液,無(wú)需配制? 稀釋后的抗體可以使用長(zhǎng)達(dá) 6 個(gè)月? 適用所有實(shí)驗(yàn)的抗體稀釋?zhuān)↖F、IHC、ELISA、WB)? 含抗體結(jié)合反應(yīng)增強(qiáng)劑、穩(wěn)定劑,效果更佳? 不含 BSA、脫脂奶粉等動(dòng)物源蛋白? 不含磷酸鹽、疊氮鈉或硫柳汞類(lèi)防腐劑運(yùn)輸和保存條件常溫運(yùn)輸,4℃存儲(chǔ),有效期兩年。操作步驟 :? 根據(jù)抗體使用說(shuō)明書(shū),直接用本品對(duì)一抗或二抗進(jìn)行適當(dāng)倍數(shù)的稀釋。? 保存在抗體稀釋液中的抗體可回收于 4℃保存,可反復(fù)使用多次,長(zhǎng)達(dá) 6 個(gè)月以上,直至不能達(dá)到理想結(jié)果后丟棄。注意事項(xiàng)1、使用后請(qǐng)旋緊瓶蓋,防止溶液揮發(fā)或與空氣的物質(zhì)發(fā)生化學(xué)反應(yīng),并放回 4℃保存。2、在進(jìn)行抗體免疫反應(yīng)前可以使用 Genefist 的高效 Western 封閉液(貨號(hào)GF1815)進(jìn)行封閉,能進(jìn)一步提高條帶的清晰度,減少顯色后的高背景和雜帶的產(chǎn)生 。3、本品不含疊氮鈉或硫柳汞類(lèi)防腐劑,但還需小心操作避免皮膚直接接觸。4、本試劑只能用于科研實(shí)驗(yàn),請(qǐng)勿用于臨床診斷和其它用途。

半干轉(zhuǎn)膜系統(tǒng)快速轉(zhuǎn)膜液(5X)Cat #. GF1816 / Size. 500 ml產(chǎn)品簡(jiǎn)介半干轉(zhuǎn)膜系統(tǒng)快速轉(zhuǎn)膜液 Western blot Rapid Transfer Buffer for semi-dry(5X)是 Genefist推出的一種 5 倍濃度的高效轉(zhuǎn)膜液,能高效快速地將蛋白轉(zhuǎn)移到印跡膜(PVDF 或硝酸纖維素膜)上。本轉(zhuǎn)膜液使用過(guò)程中,無(wú)需再使用甲醇或乙醇等任何醇類(lèi)試劑,且能在 5-15 分鐘內(nèi)完成轉(zhuǎn)膜過(guò)程,快速、高效、安全!產(chǎn)品特色:? 高效安全:不使用甲醇或乙醇等任何醇類(lèi)試劑,減少有毒試劑使用; ?? 快速穩(wěn)定:能在 5-15 分鐘完成轉(zhuǎn)膜過(guò)程,產(chǎn)熱量小,減少蛋白損失;? 兼容性好:兼容 Laemmli 膠,預(yù)制膠,F(xiàn)astPAGE,Bis-Tris 膠等多種凝膠。運(yùn)輸和保存條件:常溫運(yùn)輸,室溫存儲(chǔ),有效期一年。操作步驟 :1、1X 快速轉(zhuǎn)膜液的配制(100ml):取 20ml 快速轉(zhuǎn)膜液(5×)+ 80ml 去離子水;2、做好轉(zhuǎn)印三明治結(jié)構(gòu),然后轉(zhuǎn)移至電泳槽中,設(shè)定恒壓 20V,一般時(shí)間 5-15 分鐘完成蛋白轉(zhuǎn)膜。備注:? PVDF 膜使用前要用無(wú)水甲醇潤(rùn)濕 30 秒以上。? 轉(zhuǎn)印電流建議設(shè)定恒壓 20V,1mm 厚度的凝膠,一般 5 分鐘可以將 150kD 以下蛋白完全轉(zhuǎn)印;大于 150kD 蛋白 10-15 分鐘即可完全轉(zhuǎn)印。? 凝膠的濃度影響轉(zhuǎn)印的效率,對(duì)于膠濃度 7.5%,5-10 分鐘即可完成轉(zhuǎn)印;對(duì)于膠濃度 10%,,5-15 分鐘即可完成轉(zhuǎn)印;對(duì)于膠濃度大于 10%,建議延長(zhǎng)至 8-20 分鐘。注意事項(xiàng)1、使用本試劑前請(qǐng)仔細(xì)閱讀說(shuō)明書(shū)。2、本產(chǎn)品只能用于科研實(shí)驗(yàn),請(qǐng)勿用于臨床診斷及其它用途。

Product Information GeneFist ?series3-color High Range Protein Marker(9-245 kDa)GF6619Size:250 μl × 15 μl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume? 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.? 1.5~2.5 μl per well for general Western blottingtransfer.? Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6619 GeneFist? 3-color High Range ProteinMarker is a ready to use three-color protein standardwith 12 pre-stained proteins covering a wide range ofmolecular weights from 10 to 245 kDa in Tris-Glycinebuffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins arecovalently coupled with different chromophores foreasy identification of bands, with two referenceproteins carrying enhanced intensity correspondingto a green at 25 kDa and red at 75 kDa, respectively,as separated on SDS-PAGE (Tris-Glycine buffer).The GF6619 GeneFist? 3-color High Range ProteinMarker is designed for monitoring protein separationduring SDS-polyacrylamide gel electrophoresis,verification of Western transfer efficiency onmembranes (PVDF, nylon, or nitrocellulose) and forapproximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6619 GeneFist? 3-color High Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue2452352352Blue1801701703Blue1401301304Blue10093935Red7570726Blue6053537Blue4541428Blue3530309Green25222310Blue20181811Blue15141412Blue109103-color High Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6619 GeneFist? 3-color High Range Protein Marker resolves 12 majorbands in SDS-PAGE (Tris-Glycine, MOPS, and MESbuffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 μl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 μl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 μl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 μl × 2GF6616 GeneFist 3-color Regular Range ProteinMarker, 250 μl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 μl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 μl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 μl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 μl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 μl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 μl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 μl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 μlGF5220 GeneFist 100 bp DNA Ladder II, 500 μlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 μlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 μlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 μlGeneFist Western Marker IFor research use only

Product Information GeneFist ?series3-color High Range Protein Marker(9-245 kDa)GF6619Size:250 μl × 15 μl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume? 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.? 1.5~2.5 μl per well for general Western blottingtransfer.? Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6619 GeneFist? 3-color High Range ProteinMarker is a ready to use three-color protein standardwith 12 pre-stained proteins covering a wide range ofmolecular weights from 10 to 245 kDa in Tris-Glycinebuffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins arecovalently coupled with different chromophores foreasy identification of bands, with two referenceproteins carrying enhanced intensity correspondingto a green at 25 kDa and red at 75 kDa, respectively,as separated on SDS-PAGE (Tris-Glycine buffer).The GF6619 GeneFist? 3-color High Range ProteinMarker is designed for monitoring protein separationduring SDS-polyacrylamide gel electrophoresis,verification of Western transfer efficiency onmembranes (PVDF, nylon, or nitrocellulose) and forapproximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6619 GeneFist? 3-color High Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue2452352352Blue1801701703Blue1401301304Blue10093935Red7570726Blue6053537Blue4541428Blue3530309Green25222310Blue20181811Blue15141412Blue109103-color High Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6619 GeneFist? 3-color High Range Protein Marker resolves 12 majorbands in SDS-PAGE (Tris-Glycine, MOPS, and MESbuffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 μl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 μl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 μl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 μl × 2GF6616 GeneFist 3-color Regular Range ProteinMarker, 250 μl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 μl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 μl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 μl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 μl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 μl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 μl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 μl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 μlGF5220 GeneFist 100 bp DNA Ladder II, 500 μlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 μlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 μlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 μlGeneFist Western Marker IFor research use only

Product Information GeneFist ? series3-color Regular Range Protein Marker(9-180 kDa)GF6616Size:250 μl x 15 μl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume? 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.? 1.5~2.5 μl per well for general Western blottingtransfer.? Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6616 GeneFist? 3-color Regular RangeProtein Marker is a ready to use three-color proteinstandard with 10 pre-stained proteins covering a widerange of molecular weights from 10 to 180 kDa (9 to170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDain Bis-Tris (MES) buffer). Proteins are covalentlycoupled with a blue chromophore except for tworeference bands (one green and one red band at 25kDa and 75 kDa respectively) when separated on SDSPAGE (Tris-Glycine buffer).The GF6616 GeneFist? 3-color Regular RangeProtein Marker is designed for monitoring proteinseparation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiencyon membranes, (PVDF, nylon, or nitrocellulose) andfor approximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6616 GeneFist? 3-color Regular Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue1801701702Blue1401301303Blue10093934Red7570725Blue6053536Blue4541427Blue3530308Green2522239Blue15141410Blue109103-color Regular Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6616 GeneFist?3-color Regular Range Protein Marker resolves 10major bands in SDS-PAGE (Tris-Glycine, MOPS, andMES buffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 μl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 μl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 μl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 μl × 2GF6616 GeneFist Enhanced 3-color Regular RangeProtein Marker, 250 μl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 μl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 μl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 μl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 μl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 μl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 μl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 μl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 μlGF5220 GeneFist 100 bp DNA Ladder II, 500 μlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 μlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 μlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 μlGeneFist Western Marker IFor research use only

Product Information GeneFist ? series3-color Regular Range Protein Marker(9-180 kDa)GF6616Size:250 μl x 15 μl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume? 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.? 1.5~2.5 μl per well for general Western blottingtransfer.? Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6616 GeneFist? 3-color Regular RangeProtein Marker is a ready to use three-color proteinstandard with 10 pre-stained proteins covering a widerange of molecular weights from 10 to 180 kDa (9 to170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDain Bis-Tris (MES) buffer). Proteins are covalentlycoupled with a blue chromophore except for tworeference bands (one green and one red band at 25kDa and 75 kDa respectively) when separated on SDSPAGE (Tris-Glycine buffer).The GF6616 GeneFist? 3-color Regular RangeProtein Marker is designed for monitoring proteinseparation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiencyon membranes, (PVDF, nylon, or nitrocellulose) andfor approximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6616 GeneFist? 3-color Regular Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue1801701702Blue1401301303Blue10093934Red7570725Blue6053536Blue4541427Blue3530308Green2522239Blue15141410Blue109103-color Regular Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6616 GeneFist?3-color Regular Range Protein Marker resolves 10major bands in SDS-PAGE (Tris-Glycine, MOPS, andMES buffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 μl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 μl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 μl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 μl × 2GF6616 GeneFist Enhanced 3-color Regular RangeProtein Marker, 250 μl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 μl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 μl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 μl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 μl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 μl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 μl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 μl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 μlGF5220 GeneFist 100 bp DNA Ladder II, 500 μlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 μlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 μlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 μlGeneFist Western Marker IFor research use only