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試劑
我們的試劑品牌很多，都是高品質(zhì)的試劑
鮭魚精DNA 10mg/ml
基本售價(jià)：100.00元
鮭魚精DNA 10mg/ml產(chǎn)品簡(jiǎn)介別名單鏈DNA儲(chǔ)存條件-20℃，有效期1年規(guī)格1ml單位支Solarbio生產(chǎn)的鮭魚精DNA溶液（10mg/mL）是經(jīng)過酚氯仿抽提，超聲和熱變性處理的短片段的單鏈DNA溶液，可直接用于Southern、Northern等核酸雜交中。 本產(chǎn)品鮭魚精DNA的濃度為10mg/mL，使用時(shí)按實(shí)驗(yàn)具體要求操作，稀釋至所需工作濃度即可。 鮭魚精DNA注意事項(xiàng)： 1. 如果每次的使用量很小，可以適當(dāng)分裝后再使用，避免反復(fù)凍融。 2. 為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴手套操作。
伊文斯藍(lán)染色液(0.5%)
基本售價(jià)：150.00元
伊文斯藍(lán)染色液(0.5%)產(chǎn)品簡(jiǎn)介CAS314-13-6英文名稱Evan's Blue Stain Solution, 0.5%別名；伊文思蘭；偶氮藍(lán)；依文氏蘭；依文思藍(lán)；依文斯蘭；埃文斯藍(lán)；6,6'-[[3,3'-二甲基(1,1'-二苯基)-4,4'-二基]雙(偶氮基)]雙(4-氨基-5-羥基-1,3-萘二磺酸)四鈉鹽分子式C34H24N6Na4O14S4分子量960.81儲(chǔ)存條件室溫,避光保存,有效期1年規(guī)格100mL 500mL單位瓶伊文斯蘭(Evans Blue)又稱偶氮藍(lán)。伊文斯蘭與臺(tái)盼藍(lán)都是細(xì)胞活性染料，常用于檢測(cè)細(xì)胞膜的完整性和細(xì)胞是否存活。活細(xì)胞因有外排功能而無(wú)法被伊文斯蘭染成藍(lán)色，而死細(xì)胞會(huì)被染成淡藍(lán)色。因此可以通過此方法在顯微鏡下區(qū)分死細(xì)胞與活細(xì)胞，但無(wú)法區(qū)分死亡與壞死。備注：以上數(shù)據(jù)均來自公開文獻(xiàn), Solarbio暫未進(jìn)行獨(dú)立驗(yàn)證, 僅供參考。These protocols are for reference only. Solarbio does not independently validate these methods.
赤霉素(GA3)
基本售價(jià)：80.00元
赤霉素(GA3)產(chǎn)品簡(jiǎn)介英文名稱Gibberellic acidCAS77-06-5級(jí)別BR別名赤霉酸；赤霉素A；2,4a,7-三羥基-1-甲基-8-亞甲基赤霉-3-烯-1,10-二羧酸-1,4a-內(nèi)酯；九二零純度90.0%分子式C19H22O6分子量346.37外觀（性狀）白色粉末儲(chǔ)存條件RT有效期2年溶解性50mg/ml EtOH規(guī)格5g單位瓶赤霉素是植物生長(zhǎng)激素，能促進(jìn)莖、葉的生長(zhǎng)，提早抽苔開花，促進(jìn)種子、塊莖、塊根發(fā)芽，刺激果實(shí)生長(zhǎng),增加結(jié)果率或形成無(wú)籽果實(shí)等.結(jié)馬鈴薯、蕃茄、稻、麥、棉花、大豆、豌豆、煙草、果樹等均有增產(chǎn)作用。但施用過多的赤霉素，植物會(huì)出現(xiàn)黃而細(xì)長(zhǎng)的枝條，即失綠、徒長(zhǎng)現(xiàn)象，反而影響產(chǎn)量。赤霉素還可用于從大麥制造麥芽。它對(duì)昆蟲的發(fā)育也有促進(jìn)作用。能溶于醇、酮、酯類等有機(jī)溶劑和pH值6.3的磷酸緩沖溶液，難溶于醚、氯仿、苯和水。與鉀、鈉離子形成鹽并溶于水。赤霉素固體比較穩(wěn)定，在干燥、密閉條件下貯存時(shí)間較久不會(huì)失效；水溶液不穩(wěn)定，低溫時(shí)短期貯存不致失效，溫度較高時(shí)分解較快；酸性或弱酸性條件較穩(wěn)定，堿性條件不穩(wěn)定。 備注：產(chǎn)品信息可能會(huì)有優(yōu)化升級(jí)。請(qǐng)以實(shí)際標(biāo)簽信息為準(zhǔn)。 備注：以上數(shù)據(jù)均來自公開文獻(xiàn), Solarbio暫未進(jìn)行獨(dú)立驗(yàn)證, 僅供參考。These protocols are for reference only. Solarbio does not independently validate these methods.
維生素C
基本售價(jià)：240.00元
維生素C產(chǎn)品簡(jiǎn)介CAS50-81-7中文名稱維生素C英文名稱L-Ascorbic Acid別名L(+)-Ascorbic acid;Vitamin C;L-抗壞血酸純度HPLC≥98%分子式C6H8O6 分子量176.12外觀（性狀）White to off-white Solid儲(chǔ)存條件Powder : 2-8℃, 2 years; In solvent(母液): -20℃, 1 month; -80℃, 6 months溶解性Soluble in Water ≥20mg/mLMDLMFCD00064328ECEINECS 200-066-2InChIKeyCIWBSHSKHKDKBQ-JLAZNSOCSA-NInChIInChI=1S/C6H8O6/c7-1-2(8)5-3(9)4(10)6(11)12-5/h2,5,7-10H,1H2/t2-,5+/m0/s1PubChem CID54670067SMILESOC([C@@]1([H])[C@H](CO)O)=C(O)C(O1)=O描述L-Ascorbic Acid是一種有效的還原劑和抗氧化劑。(L-Ascorbic Acid is a potent reducing agent and antioxidant.)生物活性L-Ascorbic acid exhibits anti-cancer effects through the generation of reactive oxygen species (ROS) and selective damage to cancer cells[1].In VitroThe anti-cancer effects of L-Ascorbic acid are determined by sodium-dependent vitamin C transporter 2 (SVCT-2), a transporter of L-ascorbic acid. L-Ascorbic acid (0.1 μM-2 mM) exhibits anti-cancer effects according to SVCT-2 expression and L-ascorbic acid uptake. Human colorectal cancer cell lines displays differential responses to L-ascorbic acid, primarily depending on the expression level of SVCT-2[1].In VivoL-Ascorbic acid/Tolbutamide produces hypoglycaemic activity in a dose dependant manner in normal (60 mg/kg) and diabetic (40 mg/kg) condition. In the presence of L-ascorbic acid, Tolbuatmide (20 mg/kg) produces early onset of action and maintained for longer period compared to Tolbutamide matching control[2].數(shù)據(jù)來源文獻(xiàn)[1]. Sungrae Cho, et al. Hormetic dose response to L-ascorbic acid as an anti-cancer drug in colorectal cancer cell lines according to SVCT-2 expression. Sci Rep. 2018 Jul 27;8(1):11372.[2]. Satyanarayana Sreemantula, et al. Influence of antioxidant (L- ascorbic acid) on tolbutamide induced hypoglycaemia/antihyperglycaemia in normal and diabetic rats. BMC Endocr Disord. 2005 Mar 3;5(1):2.規(guī)格20mg 10mM*1mL (in Water) 100mg單位瓶一種重要的營(yíng)養(yǎng)素和氧化還原劑，在骨鹽代謝及骨形成中具有重要作用。在體外，它能增加鈣鹽的沉積，促進(jìn)礦化結(jié)節(jié)的形成，并通過間充質(zhì)細(xì)胞間接調(diào)節(jié)破骨細(xì)胞的分化。維生素C能直接抑制核因子κB受體活化子配體（RANKL）誘導(dǎo)的破骨細(xì)胞形成 。 使用本產(chǎn)品的應(yīng)用案例（僅供參考） In VitroCell（Osteogenic/成骨誘導(dǎo)；50 mg/l ascorbic acid） The osteogenic medium was the complete culture medium supplemented with 10 nM dexamethasone(Solarbio), 10 mM β-glycerophosphate (Solarbio), and 50 mg/l ascorbic acid (Solarbio). 數(shù)據(jù)來源文獻(xiàn)：Jia L, Zhang Y, Ji Y, Li X, Xing Y, Wen Y, Huang H, Xu X. Comparative analysis of lncRNA and mRNA expression profiles between periodontal ligament stem cells and gingival mesenchymal stem cells. Gene. 2019 May 30;699:155-164. doi: 10.1016/j.gene.2019.03.015. Epub 2019 Mar 12. PMID: 30876821. Cell（Osteogenic/成骨誘導(dǎo)；50 mg/l ascorbic acid） Osteogenic induced medium contained complete culture medium supplemented with 100 nM dexamethasone (Solarbio, Beijing, China), 10 mM β-glycerophosphate (Solarbio) and 50 mg/l ascorbic acid (Solarbio). The medium was replaced every other day. Alizarin Red–positive mineralized matrix was used to evaluate osteogenic capacity by Alizarin red (AR) staining. 數(shù)據(jù)來源文獻(xiàn)：Zhao Y, Liu H, Xi X, Chen S, Liu D. TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT. Exp Cell Res. 2020 Dec 1;397(1):112336. doi: 10.1016/j.yexcr.2020.112336. Epub 2020 Oct 19. PMID: 33091421. In Vivo Rat（雄性新生SD Rat；100 mg vitamin C/kg bw/d；灌胃；30d） Forty-five male neonatal SD rats were randomly divided into five groups (n=9 each): corn oil control group, 500 mg DEHP/kg of body weight per day (bw/d) group, 500 mg DEHP/kg bw/d+200 mg vitamin E/kg bw/d group, 500 mg DEHP/kg bw/d+100 mg vitamin C/kg bw/d group, and 500 mg DEHP/kg bw/d+200 mg vitamin E/kg bw/d+100 mg vitamin C/kg bw/d group. DEHP and vitamin E (Solarbio, China) were dissolved in corn oil, and vitamin C (Solarbio,China) was dissolved in distilled water. From postnatal day (PND) 1 to 30, the male rats were administered corn oil, DEHP, vitamin C, and/or vitamin E by gavage each day according to their group. On PND 36, six rats of each groups were sacrificed and their testes were immediately isolated. The rest of rats were fed with routine feed until PND 90. 來源文獻(xiàn)：Tang X, Wu S, Shen L, Wei Y, Cao X, Wang Y, Long C, Zhou Y, Li D, Huang F, Liu B, Wei G. Di-(2-ethylhexyl) phthalate (DEHP)-induced testicular toxicity through Nrf2-mediated Notch1 signaling pathway in Sprague-Dawley rats. Environ Toxicol. 2018 Jul;33(7):720-728. doi: 10.1002/tox.22559. Epub 2018 Apr 16. PMID: 29663635. 備注：以上數(shù)據(jù)均來自公開文獻(xiàn), Solarbio暫未進(jìn)行獨(dú)立驗(yàn)證, 僅供參考。These protocols are for reference only. Solarbio does not independently validate these methods
PerCP/Cyanine5.5 Anti-Human CD3抗體
基本售價(jià)：840.00元
PerCP/Cyanine5.5 Anti-Human CD3 Antibody[OKT-3]Uniprot : P07766 貨號(hào): E-AB-F1001J 應(yīng)用: FCM Isotype: Mouse IgG2a, κ 宿主: Mouse Antigen Details Alternate NamesT-cell surface glycoprotein CD3 epsilon chain,CD3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3ESwissprotP07766 References1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.3. Lanier L, et al. 1986. J. Immunol. 137:2501.Product Details FormLiquidClone No.OKT-3 (See Other Available Formats) HostMouseIsotypeMouse IgG2a, κIsotype ControlPerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802J] ReactivityHumanApplicationFCM (See the Application Description) Storage BufferPhosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.Recommended UseEach lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.ShippingBiological ice pack at 4℃Stability & StorageKeep as concentrated solution.Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.Centrifuge before opening to ensure complete recovery of vial contents.This product is guaranteed up to one year from purchase.ConjugationPerCP/Cyanine5.5PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).如何通過光譜圖選擇合適的檢測(cè)通道？ Background CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
豬血清（無(wú)菌過濾）
基本售價(jià)：1100.00元
豬血清（無(wú)菌過濾）貨號(hào)：S9060 保存：-20°C 有效期：5 年 產(chǎn)品說明： 本產(chǎn)品采用豬血液為原料，無(wú)菌采集、批量混合，經(jīng) 0.1μm 微孔濾芯無(wú)菌過濾分裝而成。 本品未經(jīng)過滅活，溶液狀態(tài)下為黃色澄清液體，無(wú)溶血或異物，長(zhǎng)時(shí)間靜置會(huì)有少量蛋白析出，使用前請(qǐng)震蕩混勻。 產(chǎn)品應(yīng)用（僅供參考）： 本品適合用于普通細(xì)胞培養(yǎng)，也可用于配制試劑用。 使用時(shí)需要注意以下幾點(diǎn)： 1. 血清一般儲(chǔ)存于-20℃，使用時(shí)為避免反復(fù)凍融，建議無(wú)菌環(huán)境下分裝成小包裝，儲(chǔ)存于-20℃。 2. 血清融化時(shí)最好先置于 2-8℃冰箱中使之溶解，然后在室溫下使之全溶，溶解過程中必須規(guī)則地?fù)u晃均勻。 3. 融化后的血清在 4℃不宜長(zhǎng)時(shí)間存放，應(yīng)盡快使用 注意事項(xiàng)： 1. 產(chǎn)品信息僅供參考，如有疑問請(qǐng)致電 400-968-6088 咨詢。 2. 本產(chǎn)品僅供科研使用。請(qǐng)勿用于醫(yī)藥、臨床診斷或治療，食品及化妝品等用途。請(qǐng)勿存放于普通住宅區(qū)。 3. 為了您的安全和健康，請(qǐng)穿好實(shí)驗(yàn)服并佩戴一次性手套和口罩操作。
轉(zhuǎn)鐵蛋白
基本售價(jià)：170.00元
轉(zhuǎn)鐵蛋白產(chǎn)品簡(jiǎn)介英文名稱 Transferrin CAS 11096-37-0 純度 min.98% 外觀（性狀） 白色至淺黃色固體 儲(chǔ)存條件 2-8℃ 有效期 2年 溶解性 10 mg/mL in water 規(guī)格 10mg 單位 支 產(chǎn)品說明： 轉(zhuǎn)鐵蛋白又名運(yùn)鐵蛋白 transferrin，TRF，siderophilin）是血漿中主要的含鐵蛋白質(zhì)，負(fù)責(zé)運(yùn)載由消化管吸收的鐵和由紅細(xì)胞降解釋放的鐵。以 TRF-Fe3+的復(fù)合物形式進(jìn)入骨髓中，供成熟紅細(xì)胞的生成。 轉(zhuǎn)鐵蛋白是一種能傳遞鐵離子的蛋白質(zhì)。用于生化研究。 別名：鐵傳遞蛋白 鐵蛋白 人轉(zhuǎn)鐵蛋白 純度：98% 溶解性：10 mg/mL in water 注意事項(xiàng)： 1. 我司生產(chǎn)的生化試劑如無(wú)特殊標(biāo)注，基本為非無(wú)菌包裝，若用于細(xì)胞實(shí)驗(yàn)，請(qǐng)?zhí)崆白龊妙A(yù)處理。 2. 一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。 3. 產(chǎn)品信息僅供參考，如有疑問請(qǐng)致電 400-968-6088 咨詢。 4. 本產(chǎn)品僅供科研使用。請(qǐng)勿用于醫(yī)藥、臨床診斷或治療，食品及化妝品等用途。請(qǐng)勿存放于普通住宅區(qū)。 5. 為了您的安全和健康，請(qǐng)穿好實(shí)驗(yàn)服并佩戴一次性手套和口罩操作。
總抗氧化能力(T-AOC)檢測(cè)試劑盒（FRAP法）
基本售價(jià)：200.00元
總抗氧化能力(T-AOC)檢測(cè)試劑盒（FRAP法）產(chǎn)品簡(jiǎn)介儲(chǔ)存條件2-8℃有效期6個(gè)月中文名稱總抗氧化能力(T-AOC)檢測(cè)試劑盒（FRAP法）英文名稱Total Antioxidant Capacity(T-AOC) Assay Kit（FRAP Method）檢測(cè)方法可見分光光度法可見分光光度法 Spectrophotometer單位盒規(guī)格50T/48S測(cè)定意義：測(cè)定對(duì)象中各種抗氧化物質(zhì)和抗氧化酶等構(gòu)成總抗氧化水平。在生物學(xué)、醫(yī)學(xué)和藥學(xué)研究中常常檢測(cè)血漿、血清、唾液、尿液等各種體液，細(xì)胞或組織等裂解液、植物或中草藥抽提液及各種抗氧化物(antioxidant)溶液的總抗氧化能力。 測(cè)定原理：在酸性環(huán)境下，物質(zhì)還原Fe3+-三吡啶三吖嗪(Fe3+-TPTZ)產(chǎn)生藍(lán)色的Fe2+-TPTZ的能力反映了其總抗氧化能力。 自備實(shí)驗(yàn)用品：可見分光光度計(jì)、恒溫水浴鍋、低溫離心機(jī)、1mL玻璃比色皿和蒸餾水。 相關(guān)論文：A C2H2 zinc-finger protein OsZFP213 interacts with OsMAPK3 to enhance salt tolerance in rice - PubMed (nih.gov)
大鼠白介素11(IL-11)酶聯(lián)免疫吸附測(cè)定試劑盒
基本售價(jià)：3200.00元
Rat IL-11(Interleukin 11) ELISA Kit產(chǎn)品貨號(hào):E-EL-R0562c 中文名:大鼠白介素11(IL-11)酶聯(lián)免疫吸附測(cè)定試劑盒別稱:IL11, AGIF, IL-11, interleukin 11用途 該試劑盒用于體外定量檢測(cè)Rat 血清，血漿或其他生物體液中IL-11濃度檢測(cè)原理 本試劑盒采用雙抗體夾心ELISA法。用抗大鼠IL-11抗體包被于酶標(biāo)板上，實(shí)驗(yàn)時(shí)樣品或標(biāo)準(zhǔn)品中的大鼠IL-11會(huì)與包被抗體結(jié)合，游離的成分被洗去。依次加入生物素化的抗大鼠IL-11抗體和辣根過氧化物酶標(biāo)記的親和素。抗大鼠IL-11抗體與結(jié)合在包被抗體上的大鼠IL-11結(jié)合、生物素與親和素特異性結(jié)合而形成免疫復(fù)合物，游離的成分被洗去。加入顯色底物(TMB)，TMB在辣根過氧化物酶的催化下呈現(xiàn)藍(lán)色，加終止液后變成黃色。用酶標(biāo)儀在450nm波長(zhǎng)處測(cè)OD值，IL-11濃度與OD450值之間呈正比，通過繪制標(biāo)準(zhǔn)曲線計(jì)算出樣品中IL-11的濃度。反應(yīng)類型 夾心法規(guī)格 96T反應(yīng)時(shí)間 3.5h反應(yīng)性 Rat檢測(cè)方法 Colormetric檢測(cè)范圍 15.63—1000 pg/mL靈敏度 9.38 pg/mL樣本體積 100μL樣本類型 血清，血漿或其他生物體液特異性 可檢測(cè)樣本中的大鼠IL-11，且與其它相關(guān)蛋白無(wú)明顯交叉反應(yīng)。重復(fù)性 板內(nèi)，板間變異系數(shù)均<10%。典型數(shù)據(jù) 由于實(shí)驗(yàn)操作條件的不同（如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等），標(biāo)準(zhǔn)曲線的OD值會(huì)有所差異。以下數(shù)據(jù)和曲線僅供參考，實(shí)驗(yàn)者需根據(jù)自己的實(shí)驗(yàn)建立標(biāo)準(zhǔn)曲線。(pg/mL) O.D Average Corrected 10002.4832.5052.4942.4195001.6431.6991.6711.5962500.990.9660.9780.9031250.450.4760.4630.38862.50.2740.2520.2630.18831.250.1820.170.1760.10115.630.1180.1360.1270.05200.0730.0770.075--精密度板內(nèi)精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測(cè)20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測(cè)20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 47.98127.48499.3948.54135.62511.36Standarddeviation 3.056.9720.282.997.5119.12C V (%) 6.365.474.066.165.543.74回收率 分別往5個(gè)不同樣本中添加已知濃度的目標(biāo)蛋白，做回收實(shí)驗(yàn)，得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 94-109101EDTA plasma (n=8) 96-112102Cell culture media (n=8) 90-10195線性 分別往5個(gè)樣本中添加已知濃度的目標(biāo)蛋白，做回收實(shí)驗(yàn)，得出回收率范圍及平均回收率。將5個(gè)樣本分別稀釋2倍，4倍，8倍，16倍做回收實(shí)驗(yàn)，得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)93-10795-10996-110Average (%)981021031:4Range (%)91-10979-9285-97Average (%)9985901:8Range (%)89-10280-9387-100Average (%)9686921:16Range (%)93-10679-9387-99Average (%)1008694試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周；如果一周以后才使用試劑盒，請(qǐng)拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規(guī)格 保存條件 ELISA酶標(biāo)板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃，可存放6個(gè)月凍干標(biāo)準(zhǔn)品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結(jié)合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光)，可存放6個(gè)月標(biāo)準(zhǔn)品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個(gè)月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結(jié)合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應(yīng)終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產(chǎn)品說明書Product Description1 份質(zhì)檢報(bào)告Certificate of Analysis1 份說明：所有試劑瓶蓋須旋緊以防止蒸發(fā)和微生物的污染。試劑體積以實(shí)際發(fā)貨版說明書為準(zhǔn)。相關(guān)試劑在分裝時(shí)會(huì)比標(biāo)簽上標(biāo)明的體積稍多一些，請(qǐng)?jiān)谑褂脮r(shí)量取而非直接倒出。試驗(yàn)所需自備物品 1. 酶標(biāo)儀(450nm波長(zhǎng)濾光片)2. 高精度移液器，EP管及一次性吸頭：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱，雙蒸水或去離子水4. 吸水紙操作步驟1. 對(duì)應(yīng)板孔中加入100μL標(biāo)準(zhǔn)品工作液或樣本，37℃孵育90分鐘2. 棄掉板內(nèi)液體后，立即加入100μL生物素化抗體工作液，37℃孵育60分鐘3. 棄掉板內(nèi)液體，洗板3次4. 每孔加入100μL HRP酶結(jié)合物工作液，37℃孵育30分鐘，棄掉板內(nèi)液體，洗板5次5. 每孔加入90μL底物溶液，37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長(zhǎng)下讀數(shù)，處理數(shù)據(jù)
Recombinant human IL-6 protein, C-His
基本售價(jià)：3980.00元
Recombinant human IL-6 protein, C-His產(chǎn)品編號(hào)bs-41177P英文名稱Recombinant human IL-6 protein, C-His中文名稱重組人白介素6蛋白別 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6. ;Recombinant human IL-6 protein, C-His Specific References (1) | bs-41177P has been referenced in 1 publications.[IF=4.966] 222Xiaolong Zhang. et al. Baicalein inhibits non‐small‐cell lung cancer invasion and metastasis by reducing ezrin tension in inflammation microenvironment. Cancer Sci. 2020 Oct; 111(10): 3802–3812 other ; PubMed:32691974理論分子量15kDa檢測(cè)分子量21 kDa性 狀Lyophilized or Liquid濃 度＞0.5 mg/ml物 種Human序 列30-212/212純 度>95% as determined by SDS-PAGE純化方法AC內(nèi)毒素Not analyzed表達(dá)系統(tǒng)E.coli活性Not tested標(biāo)簽C-His緩 沖 液20 mM Tris-HCl (pH=8.0) with 150 mM NaCl保存條件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事項(xiàng)This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產(chǎn)品介紹Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6 also plays a role in fighting infection, as IL-6 has been shown in mice to be required for resistance against bacterium Streptococcus pneumoniae.SWISS:P05231Gene ID:3569產(chǎn)品圖片
Recombinant mouse IL-6 protein, C-His
基本售價(jià)：1280.00元
Recombinant mouse IL-6 protein, C-His產(chǎn)品編號(hào)bs-10861P英文名稱Recombinant mouse IL-6 protein, C-His中文名稱重組小鼠白介素IL-6蛋白別 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6; Recombinant mouse IL-6 protein, C-His 理論分子量23kDa檢測(cè)分子量26 kDa性 狀Lyophilized or Liquid濃 度＞0.5 mg/ml物 種Mouse序 列25-211/211純 度>95% as determined by SDS-PAGE純化方法AC內(nèi)毒素Not analyzed表達(dá)系統(tǒng)E.coli活性Not tested標(biāo)簽C-His緩 沖 液20 mM Tris-HCl with 20% Glycerol保存條件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事項(xiàng)This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產(chǎn)品介紹This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011].SWISS:P08505Gene ID:16193產(chǎn)品圖片
Recombinant human IL-22 protein
基本售價(jià)：4800.00元
Recombinant human IL-22 protein產(chǎn)品編號(hào)bs-43572P英文名稱Recombinant human IL-22 protein中文名稱重組人白介素22蛋白性 狀Lyophilized or Liquid濃 度1mg/ml保存條件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事項(xiàng)This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產(chǎn)品介紹產(chǎn)品圖片
Recombinant human IL-10 protein (Active, HEK293)
基本售價(jià)：2080.00元
Recombinant human IL-10 protein (Active, HEK293) 產(chǎn)品編號(hào)ck0006英文名稱Recombinant human IL-10 protein (Active, HEK293)中文名稱重組人白介素10 (活性的, Tag free)別 名Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN. ;Recombinant human IL-10 protein (Active, HEK293) 人白介素10 重組蛋白; 人白細(xì)胞介素10 ; 重組人白介素10; 重組人IL-10;理論分子量17kDa檢測(cè)分子量21 kDa性 狀Lyophilized powder濃 度＞0.5 mg/ml物 種Human序 列19-178/178純 度>95% as determined by SDS-PAGE純化方法AC內(nèi)毒素<0.5 EU/μg as determined by LAL.表達(dá)系統(tǒng)HEK293 cell活性Yes標(biāo)簽Tag free緩 沖 液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存條件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.注意事項(xiàng)This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產(chǎn)品介紹Interleukin 10(IL-10) is a member of the IL-10 family of α-helical cytokines. IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of a number of proinflammatory cytokines such as IFNgamma, IL-2, IL-3, TNF and GM-CSF. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions.This recombinant human IL-10 is produced by human cells.(Tag free)Biological activity:The activity was measured by its ability to stimulate the proliferation of MC/9-2 cells (mouse master cell line).Reconstitution:Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.SWISS:P18893Gene ID:3586產(chǎn)品圖片
Recombinant human IL-15 protein (Active, HEK293)
基本售價(jià)：2080.00元
Recombinant human IL-15 protein (Active, HEK293) 產(chǎn)品編號(hào)ck0013產(chǎn)品分類重組蛋白英文名稱Recombinant human IL-15 protein (Active, HEK293)中文名稱重組人白介素15 (活性的, Tag free)英文別名rhIL-15; Interleukin-15; IL 15; Interleukin 15; Interleukin15; MGC9721; IL15; IL15_HUMAN. ;Recombinant human IL-15 protein (Active, HEK293)中文別名人白介素15重組蛋白; 重組人白介素15; 人重組白介素15;純化方法AC檢測(cè)分子量14-15 kDaGeneID3600SwissP40933濃度＞0.5 mg/ml儲(chǔ)存液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存條件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.SubunitSubcellular LocationIsoform IL15-S48AA: Secreted. Isoform IL15-S21AA: Cytoplasm. Nucleus. Note=IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.Tissue SpecificityMost abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.SimilarityBelongs to the IL-15/IL-21 family.Database linksEntrez Gene: 3600 HumanOmim: 600554 HumanSwissProt: P40933 HumanUnigene: 654378 Human多肽活性Yes蛋白質(zhì)與多肽-多肽序列49-162/162小分子偶聯(lián)蛋白-蛋白載體標(biāo)簽Tag free純度>95% as determined by SDS-PAGE內(nèi)毒素<0.5 EU/μg as determined by LAL.重組蛋白-表達(dá)系統(tǒng)HEK293 cell復(fù)溶Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Recombinant human IL-2 protein (Active, HEK293)
基本售價(jià)：1080.00元
產(chǎn)品編號(hào)ck0017產(chǎn)品分類重組蛋白英文名稱Recombinant human IL-2 protein (Active, HEK293)中文名稱重組人白介素2 (活性的, Tag free)英文別名Interleukin-2; Interleukin 2; Interleukin2; rhIL-2; T-cell growth factor; T Cell Growth Factor; Aldesleukin; IL 2; IL2; Lymphokine; POIL2; T Cell Growth Factor; TCGF; Involved in regulation of T cell clonal expansion; IL2_HUMAN. ;Recombinant human IL-2 protein (Active, HEK293)中文別名人白介素2重組蛋白; 重組人白介素2; 人重組白介素2; 重組人白介素2（IL-2）;蛋白細(xì)胞定位分泌型蛋白純化方法AC理論分子量15kDa檢測(cè)分子量17 kDaGeneID3558SwissP60568濃度＞0.5 mg/ml儲(chǔ)存液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存條件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.SubunitSubcellular LocationSecreted.DISEASENote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.SimilarityBelongs to the IL-2 family.Database linksEntrez Gene: 3558 HumanEntrez Gene: 16183 MouseEntrez Gene: 116562 RatOmim: 147680 HumanSwissProt: P60568 HumanSwissProt: P04351 MouseSwissProt: P17108 RatUnigene: 89679 HumanUnigene: 14190 MouseUnigene: 9871 Rat背景資料The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]多肽活性Yes蛋白質(zhì)與多肽-多肽序列21-153/153小分子偶聯(lián)蛋白-蛋白載體標(biāo)簽Tag free純度>95% as determined by SDS-PAGE內(nèi)毒素<0.5 EU/μg as determined by LAL.重組蛋白-表達(dá)系統(tǒng)HEK293 cell復(fù)溶Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
人白介素15(IL-15)酶聯(lián)免疫吸附測(cè)定試劑盒
基本售價(jià)：3200.00元
Human IL-15(Interleukin 15) ELISA Kit文獻(xiàn)(2) 產(chǎn)品貨號(hào):E-EL-H0222c 中文名:人白介素15(IL-15)酶聯(lián)免疫吸附測(cè)定試劑盒別稱:IL15用途 該試劑盒用于體外定量檢測(cè)Human 血清，血漿或其他生物體液中IL-15濃度檢測(cè)原理 本試劑盒采用雙抗體夾心ELISA法。用抗人IL-15抗體包被于酶標(biāo)板上，實(shí)驗(yàn)時(shí)樣品或標(biāo)準(zhǔn)品中的人IL-15會(huì)與包被抗體結(jié)合，游離的成分被洗去。依次加入生物素化的抗人IL-15抗體和辣根過氧化物酶標(biāo)記的親和素。抗人IL-15抗體與結(jié)合在包被抗體上的人IL-15結(jié)合、生物素與親和素特異性結(jié)合而形成免疫復(fù)合物，游離的成分被洗去。加入顯色底物(TMB)，TMB在辣根過氧化物酶的催化下呈現(xiàn)藍(lán)色，加終止液后變成黃色。用酶標(biāo)儀在450nm波長(zhǎng)處測(cè)OD值，IL-15濃度與OD450值之間呈正比，通過繪制標(biāo)準(zhǔn)曲線計(jì)算出樣品中IL-15的濃度。反應(yīng)類型 夾心法規(guī)格 96T反應(yīng)時(shí)間 3.5h反應(yīng)性 Human檢測(cè)方法 Colormetric檢測(cè)范圍 15.63—1000 pg/mL靈敏度 9.38 pg/mL樣本體積 100μL樣本類型 血清，血漿或其他生物體液特異性 可檢測(cè)樣本中的人IL-15，且與其它相關(guān)蛋白無(wú)明顯交叉反應(yīng)。重復(fù)性 板內(nèi)，板間變異系數(shù)均<10%。典型數(shù)據(jù) 由于實(shí)驗(yàn)操作條件的不同（如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等），標(biāo)準(zhǔn)曲線的OD值會(huì)有所差異。以下數(shù)據(jù)和曲線僅供參考，實(shí)驗(yàn)者需根據(jù)自己的實(shí)驗(yàn)建立標(biāo)準(zhǔn)曲線。(pg/mL) O.D Average Corrected 10002.4852.5412.5132.4365001.6481.6681.6581.5812500.9160.8840.90.8231250.480.50.490.41362.50.2910.2710.2810.20431.250.1790.1770.1780.10115.630.1240.1340.1290.05200.0700.0840.077--精密度板內(nèi)精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測(cè)20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測(cè)20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 47.35146.58348.0446.47145.32338.28Standarddeviation 3.028.2715.043.166.4718.37C V (%) 6.385.644.326.804.455.43回收率 分別往5個(gè)不同樣本中添加已知濃度的目標(biāo)蛋白，做回收實(shí)驗(yàn)，得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 92-10698EDTA plasma (n=8) 92-10397Cell culture media (n=8) 88-10394線性 分別往5個(gè)樣本中添加已知濃度的目標(biāo)蛋白，做回收實(shí)驗(yàn)，得出回收率范圍及平均回收率。將5個(gè)樣本分別稀釋2倍，4倍，8倍，16倍做回收實(shí)驗(yàn)，得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)96-11096-10984-97Average (%)101102901:4Range (%)88-10183-9584-96Average (%)9588911:8Range (%)91-10581-9289-99Average (%)9987941:16Range (%)93-10488-10088-100Average (%)999394試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周；如果一周以后才使用試劑盒，請(qǐng)拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規(guī)格 保存條件 ELISA酶標(biāo)板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃，可存放6個(gè)月凍干標(biāo)準(zhǔn)品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結(jié)合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光)，可存放6個(gè)月標(biāo)準(zhǔn)品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個(gè)月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結(jié)合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應(yīng)終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產(chǎn)品說明書Product Description1 份質(zhì)檢報(bào)告Certificate of Analysis1 份說明：所有試劑瓶蓋須旋緊以防止蒸發(fā)和微生物的污染。試劑體積以實(shí)際發(fā)貨版說明書為準(zhǔn)。相關(guān)試劑在分裝時(shí)會(huì)比標(biāo)簽上標(biāo)明的體積稍多一些，請(qǐng)?jiān)谑褂脮r(shí)量取而非直接倒出。試驗(yàn)所需自備物品 1. 酶標(biāo)儀(450nm波長(zhǎng)濾光片)2. 高精度移液器，EP管及一次性吸頭：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱，雙蒸水或去離子水4. 吸水紙操作步驟1. 對(duì)應(yīng)板孔中加入100μL標(biāo)準(zhǔn)品工作液或樣本，37℃孵育90分鐘2. 棄掉板內(nèi)液體后，立即加入100μL生物素化抗體工作液，37℃孵育60分鐘3. 棄掉板內(nèi)液體，洗板3次4. 每孔加入100μL HRP酶結(jié)合物工作液，37℃孵育30分鐘，棄掉板內(nèi)液體，洗板5次5. 每孔加入90μL底物溶液，37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長(zhǎng)下讀數(shù)，處理數(shù)據(jù)
人白介素23(IL-23)酶聯(lián)免疫吸附測(cè)定試劑盒
基本售價(jià)：3200.00元
Human IL-23(Interleukin 23) ELISA Kit文獻(xiàn)(10) 產(chǎn)品貨號(hào):E-EL-H0107c 中文名:人白介素23(IL-23)酶聯(lián)免疫吸附測(cè)定試劑盒別稱:IL-23A, IL23P19, P19, SGRF用途 該試劑盒用于體外定量檢測(cè)Human 血清，血漿或其他生物體液中IL-23濃度檢測(cè)原理 本試劑盒采用雙抗體夾心ELISA法。用抗人IL-23抗體包被于酶標(biāo)板上，實(shí)驗(yàn)時(shí)樣品或標(biāo)準(zhǔn)品中的人IL-23會(huì)與包被抗體結(jié)合，游離的成分被洗去。依次加入生物素化的抗人IL-23抗體和辣根過氧化物酶標(biāo)記的親和素。抗人IL-23抗體與結(jié)合在包被抗體上的人IL-23結(jié)合、生物素與親和素特異性結(jié)合而形成免疫復(fù)合物，游離的成分被洗去。加入顯色底物(TMB)，TMB在辣根過氧化物酶的催化下呈現(xiàn)藍(lán)色，加終止液后變成黃色。用酶標(biāo)儀在450nm波長(zhǎng)處測(cè)OD值，IL-23濃度與OD450值之間呈正比，通過繪制標(biāo)準(zhǔn)曲線計(jì)算出樣品中IL-23的濃度。反應(yīng)類型 夾心法規(guī)格 96T反應(yīng)時(shí)間 3.5h反應(yīng)性 Human檢測(cè)方法 Colormetric檢測(cè)范圍 39.06—2500 pg/mL靈敏度 23.44 pg/mL樣本體積 100μL樣本類型 血清，血漿或其他生物體液特異性 可檢測(cè)樣本中的人IL-23，且與其它相關(guān)蛋白無(wú)明顯交叉反應(yīng)。重復(fù)性 板內(nèi)，板間變異系數(shù)均<10%。典型數(shù)據(jù) 由于實(shí)驗(yàn)操作條件的不同（如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等），標(biāo)準(zhǔn)曲線的OD值會(huì)有所差異。以下數(shù)據(jù)和曲線僅供參考，實(shí)驗(yàn)者需根據(jù)自己的實(shí)驗(yàn)建立標(biāo)準(zhǔn)曲線。(pg/mL) O.D Average Corrected 25002.1472.1892.1682.11512501.4851.5191.5021.4496250.830.7920.8110.758312.50.3640.3740.3690.316156.250.230.2020.2160.16378.130.1480.1320.140.08539.060.0930.1050.0990.04600.0450.0610.053--精密度板內(nèi)精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測(cè)20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測(cè)20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 136.57343.261072.26129.52356.171144.30Standarddeviation 7.2015.5535.498.4820.0254.47C V (%) 5.274.533.316.555.624.76回收率 分別往5個(gè)不同樣本中添加已知濃度的目標(biāo)蛋白，做回收實(shí)驗(yàn)，得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 88-10094EDTA plasma (n=8) 94-10599Cell culture media (n=8) 87-10093線性 分別往5個(gè)樣本中添加已知濃度的目標(biāo)蛋白，做回收實(shí)驗(yàn)，得出回收率范圍及平均回收率。將5個(gè)樣本分別稀釋2倍，4倍，8倍，16倍做回收實(shí)驗(yàn)，得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)92-10795-10985-99Average (%)99102911:4Range (%)93-10484-9686-102Average (%)9890931:8Range (%)91-10683-9487-99Average (%)9989941:16Range (%)88-10384-9480-92Average (%)958987試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周；如果一周以后才使用試劑盒，請(qǐng)拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規(guī)格 保存條件 ELISA酶標(biāo)板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃，可存放6個(gè)月凍干標(biāo)準(zhǔn)品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結(jié)合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光)，可存放6個(gè)月標(biāo)準(zhǔn)品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個(gè)月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結(jié)合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應(yīng)終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產(chǎn)品說明書Product Description1 份質(zhì)檢報(bào)告Certificate of Analysis1 份說明：所有試劑瓶蓋須旋緊以防止蒸發(fā)和微生物的污染。試劑體積以實(shí)際發(fā)貨版說明書為準(zhǔn)。相關(guān)試劑在分裝時(shí)會(huì)比標(biāo)簽上標(biāo)明的體積稍多一些，請(qǐng)?jiān)谑褂脮r(shí)量取而非直接倒出。試驗(yàn)所需自備物品 1. 酶標(biāo)儀(450nm波長(zhǎng)濾光片)2. 高精度移液器，EP管及一次性吸頭：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱，雙蒸水或去離子水4. 吸水紙操作步驟1. 對(duì)應(yīng)板孔中加入100μL標(biāo)準(zhǔn)品工作液或樣本，37℃孵育90分鐘2. 棄掉板內(nèi)液體后，立即加入100μL生物素化抗體工作液，37℃孵育60分鐘3. 棄掉板內(nèi)液體，洗板3次4. 每孔加入100μL HRP酶結(jié)合物工作液，37℃孵育30分鐘，棄掉板內(nèi)液體，洗板5次5. 每孔加入90μL底物溶液，37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長(zhǎng)下讀數(shù)，處理數(shù)據(jù)
人可溶性白介素6受體(sIL-6R)酶聯(lián)免疫吸附測(cè)定試劑盒
基本售價(jià)：3200.00元
Human sIL-6R(Soluble Interleukin 6 Receptor) ELISA Kit產(chǎn)品貨號(hào):E-EL-H2518c 中文名:人可溶性白介素6受體(sIL-6R)酶聯(lián)免疫吸附測(cè)定試劑盒別稱:IL6R, CD126, IL-6R-1, IL-6RA, IL6Q, IL6RA, IL6RQ, gp80, Interleukin-6 receptor, interleukin 6 receptor用途 該試劑盒用于體外定量檢測(cè)Human 血清，血漿或其他生物體液中sIL-6R濃度檢測(cè)原理 本試劑盒采用雙抗體夾心ELISA法。用抗人sIL-6R抗體包被于酶標(biāo)板上，實(shí)驗(yàn)時(shí)樣品或標(biāo)準(zhǔn)品中的人sIL-6R會(huì)與包被抗體結(jié)合，游離的成分被洗去。依次加入生物素化的抗人sIL-6R抗體和辣根過氧化物酶標(biāo)記的親和素。抗人sIL-6R抗體與結(jié)合在包被抗體上的人sIL-6R結(jié)合、生物素與親和素特異性結(jié)合而形成免疫復(fù)合物，游離的成分被洗去。加入顯色底物(TMB)，TMB在辣根過氧化物酶的催化下呈現(xiàn)藍(lán)色，加終止液后變成黃色。用酶標(biāo)儀在450nm波長(zhǎng)處測(cè)OD值，sIL-6R濃度與OD450值之間呈正比，通過繪制標(biāo)準(zhǔn)曲線計(jì)算出樣品中sIL-6R的濃度。反應(yīng)類型 夾心法規(guī)格 96T反應(yīng)時(shí)間 4.5h反應(yīng)性 Human檢測(cè)方法 Colormetric檢測(cè)范圍 15.63—1000 pg/mL靈敏度 9.38 pg/mL樣本體積 100μL樣本類型 血清，血漿或其他生物體液特異性 可檢測(cè)樣本中的人sIL-6R，且與其它相關(guān)蛋白無(wú)明顯交叉反應(yīng)。重復(fù)性 板內(nèi)，板間變異系數(shù)均<10%。典型數(shù)據(jù) 由于實(shí)驗(yàn)操作條件的不同（如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等），標(biāo)準(zhǔn)曲線的OD值會(huì)有所差異。以下數(shù)據(jù)和曲線僅供參考，實(shí)驗(yàn)者需根據(jù)自己的實(shí)驗(yàn)建立標(biāo)準(zhǔn)曲線。(pg/mL) O.D Average Corrected 10002.4172.4692.4432.3845001.6951.7411.7181.6592500.9740.9390.9570.8981250.4350.4550.4450.38662.50.2680.2460.2570.19831.250.1750.1490.1620.10315.630.1060.1180.1120.05300.0490.0690.059--精密度板內(nèi)精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測(cè)20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測(cè)20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 54.13124.28477.0256.05115.03491.14Standarddeviation 3.265.4422.943.755.2221.71C V (%) 6.024.384.816.694.544.42回收率 分別往5個(gè)不同樣本中添加已知濃度的目標(biāo)蛋白，做回收實(shí)驗(yàn)，得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 83-9590EDTA plasma (n=8) 87-9792Cell culture media (n=8) 83-9590線性 分別往5個(gè)樣本中添加已知濃度的目標(biāo)蛋白，做回收實(shí)驗(yàn)，得出回收率范圍及平均回收率。將5個(gè)樣本分別稀釋2倍，4倍，8倍，16倍做回收實(shí)驗(yàn)，得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)87-9998-11196-110Average (%)931051031:4Range (%)86-100101-11698-111Average (%)921071041:8Range (%)84-9797-10894-111Average (%)891021021:16Range (%)83-92100-11792-107Average (%)88108100試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周；如果一周以后才使用試劑盒，請(qǐng)拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規(guī)格 保存條件 ELISA酶標(biāo)板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃，可存放6個(gè)月凍干標(biāo)準(zhǔn)品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結(jié)合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光)，可存放6個(gè)月標(biāo)準(zhǔn)品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個(gè)月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結(jié)合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應(yīng)終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產(chǎn)品說明書Product Description1 份質(zhì)檢報(bào)告Certificate of Analysis1 份說明：所有試劑瓶蓋須旋緊以防止蒸發(fā)和微生物的污染。試劑體積以實(shí)際發(fā)貨版說明書為準(zhǔn)。相關(guān)試劑在分裝時(shí)會(huì)比標(biāo)簽上標(biāo)明的體積稍多一些，請(qǐng)?jiān)谑褂脮r(shí)量取而非直接倒出。試驗(yàn)所需自備物品 1. 酶標(biāo)儀(450nm波長(zhǎng)濾光片)2. 高精度移液器，EP管及一次性吸頭：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱，雙蒸水或去離子水4. 吸水紙操作步驟1. 對(duì)應(yīng)板孔中加入100μL標(biāo)準(zhǔn)品工作液或樣本，37℃孵育90分鐘2. 棄掉板內(nèi)液體后，立即加入100μL生物素化抗體工作液，37℃孵育60分鐘3. 棄掉板內(nèi)液體，洗板3次4. 每孔加入100μL HRP酶結(jié)合物工作液，37℃孵育30分鐘，棄掉板內(nèi)液體，洗板5次5. 每孔加入90μL底物溶液，37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長(zhǎng)下讀數(shù)，處理數(shù)據(jù)
未包被人白介素6(IL-6)酶聯(lián)免疫吸附測(cè)定試劑盒
基本售價(jià)：4800.00元
Uncoated Human IL-6(Interleukin 6) ELISA KitUniprot : P05231 中文名稱:未包被人白介素6(IL-6)酶聯(lián)免疫吸附測(cè)定試劑盒產(chǎn)品貨號(hào): E-UNEL-H0097 實(shí)驗(yàn)類型: Sandwich-ELISA Applications:ELISA 產(chǎn)品用途 Elabscience?新系列未包被(Uncoated)ELISA試劑盒，包含用于ELISA實(shí)驗(yàn)所需的基本組分。對(duì)于具有豐富經(jīng)驗(yàn)，可自行建立ELISA實(shí)驗(yàn)體系的客戶，未包被試劑盒是其節(jié)省成本的理想選擇。可用于體外定量檢測(cè)人血清，血漿中IL-6濃度。產(chǎn)品屬性 反應(yīng)類型夾心法規(guī)格96T*5 96T*15反應(yīng)時(shí)間24h反應(yīng)性人保存條件-20℃。請(qǐng)按照說明書要求儲(chǔ)存試劑盒。樣本類型血清，血漿樣本體積100μL產(chǎn)品組成 中文名稱 英文名稱 規(guī)格 保存條件 ELISA預(yù)制酶標(biāo)板(可拆卸)Micro ELISA pre-Plate(Dismountable)96T*5：5 塊 96T酶標(biāo)板-20℃，可存放6個(gè)月凍干標(biāo)準(zhǔn)品Reference Standard96T*5：1支濃縮包被抗體Concentrated Capture Ab96T*5：1 支 120μL濃縮生物素化抗體Concentrated Biotinylated Detection Ab96T*5：1 支 120μL濃縮HRP酶結(jié)合物Concentrated HRP Conjugate96T*5：1 支 120μL-20℃(避光)，可存放6個(gè)月產(chǎn)品說明書Product Description1 份/質(zhì)檢報(bào)告Certificate of Analysis1 份實(shí)驗(yàn)原理 1. 預(yù)制酶標(biāo)板包被上分析物特異性抗體(包被抗體)，未結(jié)合的包被抗體被洗脫。后完成封閉操作，得到預(yù)包被酶標(biāo)板。2. 加入的樣本或標(biāo)準(zhǔn)品將被固定的包被抗體所捕獲，未被捕獲的成分將被移除。3. 加入的生物素化檢測(cè)抗體將結(jié)合已被包被抗體捕獲的分析物，未結(jié)合的成分將被洗脫。4. 加入HRP酶結(jié)合物結(jié)合生物素化檢測(cè)抗體，未結(jié)合的成分將被洗脫。5. 加入TMB底物溶液，藍(lán)色與樣品中存在的分析物的量成比例。加入終止液后，底物顯色變化終止，孔內(nèi)顏色由藍(lán)色變成黃色。測(cè)量450nm處各孔的吸光度值。
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